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Summary Attributions

Clone Name: sim1-865 Gene: sim1 Species: Xenopus tropicalis  
Description: EXRC Clone Number 865. Xenopus tropicalis single-minded homolog 1. Image EST 7563377. To generate probe for in situ.


5' EST: CX468007 [+]

3' EST: CX468006 [+]


Name: NIH_XGC_tropGas7External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 ( Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).

Anatomy: whole organismStage: NF stage 10.5 to NF stage 12.5
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCS108

Type: plasmid

5' Restriction Site: SalI

3' Restriction Site: NotI
Expression Image


Whole mount in situ hybridization: Expressed in somite and muscle.  
Type Size Promoter Linearization Site Description
antisense T7 SalI EcoRI and ClaI can also be used.


Search for clone at: EXRC