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Fig. 7.
The localizations of P2Y1 receptor in muscles and motor neurons. A, Chick, rat, orXenopus muscle section (20 μm) was used. For each, the same field is shown stained by the anti-P2Y1-receptor antibody (green) or for AChR (red) by TMR-BuTX (10 nM) or superimposed (yellow). Controls are noted in Results. Scale bar, 20 μm. B, Spinal cord from adult chicken. Peroxidase-conjugated secondary antibody was used here to reveal (brown) the P2Y1 receptor sites in the ventral horn. In the control, the antibody was pretreated with an excess of the recombinant P2Y1 receptor antigen. A high-power magnification is shown for two adjacent positive cells seen in the stained low-power field. Scale bars, 500 μm (low power); 100 μm (high power). |
