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Fig. 4. RNase protection analysis of APP mRNA in Xenopus tissues. Radiolabeled anti-sense RNA (nt 1–591 of X-APP-A) was transcribed from Xenopus APP–cDNA and hybridized to 5 μg of total RNA extracted from brain (Br), heart (He), lung (Lu), liver (Li), kidney (Ki), spleen (Sp), muscle (Mu), oocyte (Oo), ovary (Ov), testis (Te) and intestine (In). Following RNase treatment, samples were loaded onto a denaturing polyacrylamide gel and the dried gel was analyzed with a phosphor-imager (BioRad). Undigested probe (C1) or probe hybridized to 20 μg of yeast tRNA prior to digestion (C2) served as the controls for the specificity of the protected fragments. |