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Figure 2. The expression pattern of par6b mRNA and the subcellular localization of Par6b protein during development. (A) Sagittal view of par6b expression pattern in a st9 blastula embryo by whole mount ISH. (B) Sense par6b probe control in a st 9 embryo. (C) Transverse view of par6b expression pattern in a st 17 neurula embryo. (D) par6b expression pattern in a transverse section of st17 embryo. (E) Staining of E-cadherin in the epidermis in a transverse section of st17 embryo. (I–N) Staining with anti-HA of Par6b (red) and anti-β-catenin (green) antibodies on st9 or st17 ectoderm. (D–N). Double-headed arrows (black in panel D and E, white in panel I–N) respectively indicate boundaries of the superficial and deep layers. Scale bars, 50 µm.
doi:10.1371/journal.pone.0076854.g002 |