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Figure 8. Hif-1α/Twist are repressors of E-cadherin in the neural crest. (A–L) Immunofluorescence against E-cadherin performed in neural crest cultured in vitro. (M–S) Histological sections of immunofluorescence against E-cadherin performed in whole embryos. The diagram shows the orientation of the sections. White outlines show epidermis and placodes; green outlines show three streams of cephalic neural crest; e, eye. Red staining shows E-cadherin; blue staining shows DAPI; purple staining in P and S shows cells injected with ATGMOhif-1α. Arrows show neural crest. Note that some of the neural crests injected with the MO show E-cadherin staining (arrows in Q). (T and U) Temporal analysis of E-cadherin expression. Prospective neural crest (stage 13) and premigratory neural crest (stage 16) were dissected, and quantitative PCR was performed for E-cadherin and EF-1α (loading control). Bars represent the standard deviation from three independent experiments. ***, P < 0.005. NC, neural crest; qPCR, quantitative PCR; St, stage. |
