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Fig. 2. E-cadherin is expressed in CNC in vivo. (A) Schematics of the experimental approach for labelling CNC cells in whole embryo sections: Xenopus embryo was injected with H2B GFP mRNA and labelled CNC cells were transplanted into another unlabelled wild type host embryo. Transplanted embryos were fixed at stage 25 and transverse sectioned. (B–H) Immunofluorescence staining against endogenous E-cadherin: (B) Mandibular, hyoid and branchial subpopulation of CNC were labelled by H2B GFP in whole embryo section. Cropped image of mandibular (C,D), hyoid (E,F) and branchial (G,H) showed in detail E-cadherin expression at cell–cell contacts. Dashed orange lines indicate the boundaries between epithelia and CNC. Arrows show the staining of E-cadherin in epithelia, arrowheads indicate the staining of E-cadherin in CNC. α-E-cad: anti-E-cadherin antibody; H2B GFP: CNC injected with H2B GFP mRNA; BF: bright field; ma: mandibular; hy: hyoid; br: branchial; Epi: epithelia. Scale bar: 20 μm. |
