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cdh1xenopus   

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Experiment details for cdh1

Development of Xenopus laevis bipotential gonads into testis or ovary is driven by sex-specific cell-cell interactions, prol...

Development of Xenopus laevis bipotential gonads into testis or ovary is driven by sex-specific cell-cell interactions, proliferation rate, cell migration and deposition of extracellular matrix.

Gene Clone Species Stages Anatomy
cdh1.L laevis NF stage 49 to NF stage 52 epithelium , gonad
cdh1.L laevis NF stage 58 ovary , epithelium

  Fig. 3. Immunolocalization of E-cadherin marks somatic cells derived from coelomic epithelium. A. Undifferentiated gonads at NT49; E-cadherin is present in surface epithelium (se) but not in stromal cells (st). B. Undifferentiated gonads at NF52; E-cadherin is present in the gonadal surface epithelium (se) and medulla (m) but not in stroma (st). C. The ovary at NF58; E-cadherin is present in cortex and epithelium (e) lining ovarian secondary cavity (cav) originating from the medulla, E-cadherin is absent in the stroma (st). D. Sterile ovary at NF58 clearly shows that surface epithelium (se) and epithelium lining ovarian secondary cavity are positive for E-cadherin (indicating common origin), and stroma (st) is E-cadherin negative. E. Testes at NF57; E-cadherin is present mainly in the somatic cells enclosing spermatogonia (pre-Sertoli cells, pSc), lower E-cadherin signal is present in surface epithelium, and there is no E-cadherin in the stroma (st). F. Negative control; undifferentiated gonads at NF52. Scale bar 20 µm.