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Fig. 8. Larval and adult Xenopus epithelia stained with E-cadherin (E-cad) antibody. (A–D) Confocal images showing uniform distribution of E-cad (green) in the membranes of epithelial cells in a larval Xenopus cornea. (A′-D′) Merged images for A-D with Hoechst labeled nuclei (magenta). (A-A′) E-cad localizes to the membranes in apical cells of the central corneal epithelium. (B-B′) Basal cells of the central corneal epithelium show E-cad expression predominantly associated with the membranes. Weak E-cad expression is also detected in the cytoplasm of the basal cells. (C-C′) Membrane bound E-Cad in apical cells of the peripheral corneal epithelium. (D-D′) E-cad labeling is associated with the membranes of basal epithelial cells of the peripheral cornea. (E–H) Fluorescence light microscopic images showing E-cad (green) staining on frozen sections of adult Xenopus epithelia, as labeled. The apical surface is located towards the top of each image, and the basal surface towards the bottom. (H′-K′) Merged images for H-K with Hoechst labeled nuclei (magenta). (E-E′) Epithelial cells throughout the entire thickness of the central cornea have uniform expression of E-cad in the membrane. (F-F′) E-cad localizes to the membrane of all epithelial cells in the limbal region. (G-G′) E-cad uniformly labels the membranes of epithelial cells in the inner and outer surface of the ventral eyelid. (H-H′) Cross-section of skin epithelium with E-cad membrane associated staining in the basal, intermediate and superficial layers. ive, inner ventral eyelid; ove, outer ventral eyelid. Scale bar in H′ equals 25 μm for A-D, A′-D′, and 50 μm for E-H, E′-H’. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.) |
