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cdh1xenopus superficial 

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Experiment details for cdh1

Par6b Regulates the Dynamics of Apicobasal Polarity during Development of the Stratified Xenopus Epidermis.

Par6b regulates the dynamics of apicobasal polarity during development of the stratified Xenopus epidermis.

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Gene Clone Species Stages Anatomy
cdh1.L laevis NF stage 17 superficial

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  Figure 6. Par6b depletion stabilizes Crb3 on deep cell membranes, and destabilizes Lgl2 in both epidermal layers. (A) Crb3-GFP signal in a transverse section of st17 embryos. 25 ng of Par6b-MO was injected into one blastomere at the 2-cell stage. Left half is the uninjected side and right half is the Par6b-MO injected side. (B) Crb3-GFP distribution in uninjected epidermis, left box in figure A. (C) Crb3-GFP distribution in Par6b-depleted side, right box in figure A. (D, E) GFP-Lgl2 signal in transverse section of st12.5 and st17 injected embryos. 10 ng of Par6b-MO with RIDX was injected into one animal ventral blastomere at the 8-cell stage. (E) Western blot of total protein extracts from st12.5 and st16/17 Crb3-GFP or GFP-Lgl2 injected host transfer embryos with or without injection of 50 ng Par6b-MO. doi:10.1371/journal.pone.0076854.g006