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cdkn1axenopus   

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Experiment details for cdkn1a

Identification of Xenopus cyclin-dependent kinase inhibitors, p16Xic2 and p17Xic3.

Identification of Xenopus cyclin-dependent kinase inhibitors, p16Xic2 and p17Xic3.

Gene Clone Species Stages Anatomy
cdkn1a.L laevis NF stage 7 animal hemisphere
cdkn1a.L laevis NF stage 18 to NF stage 25 cement gland , cement gland primordium , somite
cdkn1a.L laevis NF stage 32 lens , presomitic mesoderm , somite , tail bud , lens placode , [+]

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  Fig. 3. Expression patterns of p16Xic2 and p17Xic3 during Xenopus development. (A) Semiquantitative RT-PCR analysis of p16Xic2 and p17Xic3 during Xenopus development. cDNAs were made from total mRNAs isolated from the corresponding stages. After determination of the best conditions for semiquantitative analysis, RT-PCR was performed. ODC was used as a control. (B–J) Whole mount in situ hybridization of p16Xic2. (B) stage 7; (C) anterior view, stage 18; (D) lateral view, stage 23; (E) dorsal view, stage 23; (F) anterior view, stage 25; (G) lateral view, stage 25; (H) lateral view, stage 29/30; (I) lateral view, stage 32; (J) a section of retina at stage 32; (K) whole mount in situ hybridization of p17Xic3 at stage 33.