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cdkn1axenopus axial mesoderm [+] 

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Experiment details for cdkn1a

Klisch TJ et al. (2006) Assay

Mxi1 is essential for neurogenesis in Xenopus and acts by bridging the pan-neural and proneural genes.

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Gene Clone Species Stages Anatomy
cdkn1a.L laevis NF stage 14 axial mesoderm

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  Fig. 6. Xmxi1 inhibits genes required for cell cycle withdrawal but does not influence neurogenic and early proneural genes. (A–G) Whole-mount in situ hybridization of stage 14 embryos injected with 500 pg of MT-Xmxi1. Antisense probes used are indicated in the lower left hand corner. The injected side is always on the right, and all embryos are shown as dorsal views, anterior down. (H) Transversal section of G. (I) Xmxi1 blocks induction of late, but not early X-ngnr-1-induced target genes in animal caps. Real-time RT-PCR analysis of animal cap explants isolated from embryos injected with X-ngnr-1 (25 pg) alone or together with MT-Xmxi1 (500 pg). Expression levels were normalized to ODC and compared to the induction capacity of X-ngnr-1 injected animal caps, which were set to 10.