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Fig. 2. Inhibition of miRNA biogenesis affects patterning of the pronephros. (A-G′) Whole-mount in situ hybridization for markers of terminal pronephros differentiation on uninjected and Dgcr8-MO-injected Xenopus embryos at stage 39. (A,A′) SGLT1-K; (B,B′) NKCC2; (C,C′) NBC1; (D,D′) ClC-K; (E,E′) Cadherin-16; (F,F′) ROMK; (G,G′) NCC. Arrowheads indicate a reduction in the intermediate and distal tubular domain. (H,I) Schematics illustrating the different pronephric regions and their corresponding marker gene expression (H) and the phenotype of Dicer-MO- or Dgcr8-MO-injected embryos showing a reduction in the proximal and intermediate tubules as well as in distal tubule DT1 (I). |
