|
Display additional annotations [+]
|
|
Figure 10. Whole-mount in situ hybridization assays at early stages illustrating common expression patterns of 10 down-regulated genes: IMAGE 4174145, IMAGE 3399268, IMAGE 4057931, IMAGE 3400511, Socs3, MGC114680, IMAGE 7010138, Cnfn1-a, Ralgds, Dnaja4.2. For all genes, expression is enhanced in the animal cells (an) of cleavage and blastula embryos, and is throughout the ectoderm at gastrula stages (side views). At early neural plate stages, all genes are expressed in the epidermis (epi), often more intensely on the dorsal (d) side, including neural plate (np) and border zone (bz) (all except IMAGE 3400511 are side views with anterior [a] to the left; IMAGE 3400511 is an anterior view). At later neural plate and neural tube stages, expression is strongest in the neural crest (nc), pre-placodal region (ppr), neural tube (nt) and epidermis (epi) (anterior views). Cnfn1-a is uniquely expressed in the hatching gland (hg). |
|
Display additional annotations [+]
|
|
Figure 8. Examples of embryos bisected after the in situ hybridization protocol to reveal expression patterns in internal cells. BQ399495: At blastula (left image, sagittal section), expression is primarily in the animal cap (AC) cells, and barely detected in the equatorial (eq) zone. At neural plate stages (right image, transverse section), expression is observed in the neural plate (np), somites (so), and epidermis (epi). Hprt1: At blastula (left image, sagittal section), expression is in both animal cap and equatorial cells. At gastrula stages (right image, sagittal section, anterior to the left), expression is observed in the surface ectoderm (ecto) and involuting mesoderm (meso); bl, dorsal blastoporal lip. Xxylt1: At blastula (left image, sagittal section), expression is in both animal cap and equatorial cells. At neural plate stages (right image, sagittal section, anterior to the left), expression is observed in the neural plate and underlying dorsal mesoderm (meso). IMAGE 3399268: At blastula (left image, sagittal section), expression is in both animal cap and equatorial cells. At gastrula stages (right image, sagittal section, anterior to the left), expression is observed in the animal ectoderm, but barely detected in the involuting mesoderm. Cnfn1-a: At gastrula stages (sagittal section, anterior to the left), expression is observed in the animal ectoderm, but not in the involuting mesoderm. IMAGE 7010138: At blastula (left image, sagittal section), expression is primarily in the animal cap cells, and barely detected in the equatorial zone. At gastrula stages (middle image, sagittal section, anterior to the left), there is strong expression in both the ectoderm and involuting mesoderm. At neural plate stages (right image, sagittal section, anterior to the left), weak expression is observed in both the neural plate and underlying dorsal mesoderm. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
cnfn.1
|
|
laevis
|
NF stage 24
to
NF stage 33 and 34
|
otic vesicle
,
hatching gland
,
rectal diverticulum
|
dnaja4.2
|
|
laevis
|
NF stage 24
to
NF stage 33 and 34
|
otic vesicle
,
lens
,
brain
,
pharyngeal arch
|
septin5
|
|
laevis
|
NF stage 24
to
NF stage 33 and 34
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
cranial nerve
,
[+]
|
socs3
|
|
laevis
|
NF stage 24
to
NF stage 33 and 34
|
otic vesicle
,
lens
,
retina
,
pharyngeal arch
,
mandibular arch
,
[+]
|
ralgds
|
|
laevis
|
NF stage 24
to
NF stage 33 and 34
|
spinal cord
|
fam89a
|
|
laevis
|
NF stage 24
to
NF stage 33 and 34
|
lens
,
brain
,
cranial ganglion
,
trigeminal ganglion
,
pronephric kidney
,
[+]
|
|
|
Figure 11. Whole-mount in situ hybridization assays at tail bud to larval stages illustrating more divergent expression patterns of 10 down-regulated genes: IMAGE 4174145, IMAGE 3399268, IMAGE 4057931, IMAGE 3400511, Socs3, MGC 114680, IMAGE 7010138, Cnfn1-a, Ralgds, Dnaja4.2. Side views at tail bud (left column) and larval (middle column) stages, with anterior to the left and dorsal to the top, and transverse sections at larval stages (right side) with dorsal to the top. Abbreviations are as in Figure 10. For Cnfn1-a: hg, hatching gland; clo, cloaca. |