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Fig. 5. Comparison of XSip1, XCtBP, and XCtBP1 expression during embryogenesis. (A) RT-PCR analysis of XCtBP, XCtBP1, and XSip1 expression at the indicated embryonic stages. Histone H4 was used as a loading control. Note that both XCtBP and XCtBP1 RNA are detected throughout development, while XSip1 transcripts accumulate at the start of zygotic transcription. (B) Whole-mount in situ hybridization of XCtBP, XCtBP1, and XSip1 at the indicated stages. All embryos are shown with anterior toward the right. (C) XCtBP expression in a horizontal section of a stage 18 embryo with a blow-up showing the restricted XCtBP expression in the deep sensorial layer of the ectoderm. Transversal sections of the neural tube at the level of the hindbrain of stage 31 embryos are shown at the right. Note that XCtBP and XCtBP1 have similar expression patterns during early embryonic development and are co-expressed with XSip1 in the developing neural tissue, XCtBP being expressed earlier and at a higher level than XCtBP1. Later, XCtBP and XCtBP1 have distinct expression domains within the developing neural tube. Abbreviations: mz, marginal zone; vz, ventricular zone; pn, pronephros; ba, branchial arches; nc, neural crest; nt, neural tube; pm, posterior mesoderm; sl, sensorial layer of the ectoderm. St. 11 embryos: dorso-vegetal view, anterior right; St. 13 embryos: dorsal view, anterior right; St. 25 and 31 embryos: lateral view, anterior right. |
