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Fig. 1. Dystroglycan gene expression during early Xenopus laevis retinogenesis. Panels A�D are sections of in situ whole-mount hybridized embryos from stage 19
to stage 28. (A) Tangential section showing DG mRNA expression localized in the anterior neural plate at stage 19. (B�D) Transversal sections showing X-DG
mRNA expression at stage 24 (B), at stage 26 (C) and at stage 28 (D) in the optic stalk, in the presumptive pigmented epithelium and in the lens vesicle (C, D).
Panels E� IV are cryostat sections showing. X-DG immunolocalization. (E) X-DG detection at stage 28 in the neural tube and in the retina at the level of the
presumptive pigmented epithelium, the optic stalk and all around the sensorial layer of the ectoderm. (F, G) High magnification of retina sections at stage 32 showing
the presence of X-DG in the endfeet of retinal precursors in the ciliary marginal zone and in the inner limiting membrane at the vitreal border of the retina (purple
arrowheads in panel G). Green arrowheads indicate X-DG detection in the lens vesicle. (H� IV) Transverse sections of Xenopus retina at stage 45 showing X-DG
immunoreactivity at the level of retinal pigmented epithelium (white arrowhead), inner limiting membrane (purple arrowhead) and lens epithelium (green
arrowhead). Punctuated distribution of X-DG in the OPL is evidenced at high magnification in panels I, IV (same section, red detection) between the two nuclear
layers, highlighted in panel IV by Hoechst staining. Abbreviations: anp, anterior neural plate; OS, optic stalk; ppe, presumptive pigmented epithelium; nr, neural
retina; nrp, neural progenitors; lv, lens vesicle; d, dorsal; v, ventral; nt, neural tube; sle, sensorial layer of ectoderm; cmz, ciliary marginal zone; gcl, ganglion cell
layer; inl, inner nuclear layer; onl, outer nuclear layer; opl, outer plexiform layer; ipl, inner plexiform layer; ilm, inner limiting membrane. |
