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Supplemental Figure 8. GRAF depletion does not affect somite rotation or myoseptal alignment but
leads to progressive loss of segmental integrity. A,B) Laser scanning confocal microscopy and digital
deconvolution of whole-mount stage 25 Con Mo- and GRAF Mo-injected embryos stained with designated
antibodies. Note appropriate alignment of nuclei at the mid-point of organized myoseptal boundaries
(demarcated by LAM, -dystroglycan, and paxillin). Panel B shows example of mild phenotype observed in
3/25 Stage 25 GRAF morphants. C. Stage 37 Con Mo- and GRAF Mo-injected embryos co-stained with -
dystroglycan (green) and TOPRO (nuclei, red) reveals progressive disruption of myoseptal boundaries in late
stage GRAF morphants. |
