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dag1xenopus   

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Experiment details for dag1

Hidalgo M et al. (2009) Assay

In vivo analyzes of dystroglycan function during somitogenesis in Xenopus laevis.

Gene Clone Species Stages Anatomy
dag1 laevis NF stage 21 presomitic mesoderm , mesonephric duct , pronephric kidney , neural tube
dag1.L laevis NF stage 21 presomitic mesoderm , paraxial mesoderm , pronephric kidney
dag1.L laevis NF stage 23 brain , presomitic mesoderm , pharyngeal arch , otic placode , mesonephric duct , [+]
dag1.L laevis NF stage 27 otic vesicle , brain , presomitic mesoderm , somite , visceral pouch , [+]
dag1.L laevis NF stage 40 notochord , intersomitic region

  Figure 1. Dystroglycan transcript and protein expressions. A-C: Lateral view, whole-mount in situ hybridization at stage 21 (A), 23 (B), and 27 (C). Dg mRNAs are detected in the dorsal region, posteriorly in the presomitic mesoderm and in the mesoderm in a series of stripes. D: Stage 40, lateral view, in toto immunodetection. The staining of the Dg protein is associated with the intersomitic junctions and the notochord (white arrow). mu m; b, brain; ov, otic vesicle; psm, presomitic mesoderm; s, somites; va, visceral arches; p, pronephros and duct. Scale bar = 400 mu m.