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Fig. 3. Expression of X-dg during Xenopus development (A). Whole-mount in situ hybridization was carried out using digoxigenin-labeled cRNA probe.
Nieuwkoop-Faber stages of embryogenesis are indicated in higher left corners. Dorsal views, anterior down (A, B, E); postero-lateral view (C); anterior views
(D, F, H); lateral views, anterior on the right (G, I). Transverse section of stage 22 (J): arrow head indicates sensorial layer of the ectoderm; parasagittal section
of stage 22 (K) shows strong expression in the eye anlage and in the diencephalon/mesencephalon regions. X-dg expression sites at stage 33 (arrow heads in I):
central nervous system (black), heart (orange), lens epithelium (turquoise), mandibular, hyoid and branchial arches (yellow and green respectively), myotomes
(purple), otic vesicle (red), pronephron (blue). Parasagittal and longitudinal sections of stage 33 showing X-dg transcripts (L) and protein localization (M) in the
myotomes. Transverse section of adult skeletal muscle reveals protein localization in the sarcolemma (N). |
