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dag1xenopus anterior 

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Experiment details for dag1

Bello V et al. (2008) Assay

A function for dystroglycan in pronephros development in Xenopus laevis.

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Gene Clone Species Stages Anatomy
dag1 laevis NF stage 23 anterior

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  Fig. 1. Dystroglycan expression during Xenopus laevis pronephros development. Panels A–D show spatial expression of dystroglycan transcripts from stage 23 to 35, and schematic representation of the developing pronephros adapted from Vize et al. (1995). Whole-mount in situ hybridizations were performed using digoxigenin-labelled antisense αDg RNA probes. (A) Stage 23. Expression is confined to the developing pronephric tubule anlage. (B–D) Stage 28, 31 and 35. Dg transcripts are present in the nephrostomes, the pronephric tubules (red arrowhead) and in the pronephric duct (green arrowhead). Panels E–H show immunolocalization of Dg protein. Embryonic stages are indicated in the top right of each panel. (E) Stage 35. Dg is present in the pronephric tubules (red arrowhead), in the anterior and posterior segments of the pronephric duct (blue and green arrowheads). (F–H) Cryostat sections. (F) Stage 29/30. Dg protein is detected on the surface of cells into the pronephric bud (yellow arrowhead) and at the basal pole of cells surrounding the pronephric bud (white arrowhead). (G) Stage 36. The Dg is detected around the nephrostomes (yellow arrowhead) and the pronephric tubules (red arrowhead). (H) Stage 40. The Dg is present in the elongating tubules (white arrowhead), and duct (yellow arrowhead).