| |
Fig. 2. Expression of CXCL14 by whole-mount in situ hybridization. (A) Onset of CXCL14 expression at stage 20 in the prospective cement gland anterior to the neural plate (black arrow). Frontal view, dorsal to top. (B-D) At stage 23 CXCL14 is detected in part of the cement gland (black arrows), the dorsal anterior portion of the optic vesicle (red arrows) and the hatching gland (white arrows). (B) Lateral view, dorsal to top, anterior to right. (C) Ventral view, anterior to right. (D) Frontal view, dorsal to top. The expression of Xhe (E) and Pax3 (F) in the hatching gland (white arrows) at this stage are shown for comparison. (E-F) Frontal view, dorsal to top. (G-I) CXCL14 expression at stage 28. In addition to the cement gland (black arrows), optic vesicle (red arrows) and hatching gland (white arrows), CXCL14 is also detected in the otic vesicle (green arrows). (G) Lateral view, dorsal to top, anterior to right. (H) Dorsal view, anterior to right. (I) Ventral view, anterior to right.(J) The expression pattern of Trp2 in the pigmented epithelium of the retina is similar to CXCL14 expression in the developing eye (red arrow). Lateral view, dorsal to top, anterior to right. (K-L) Transverse section highlights the expression of CXCL14 expression in the pigmented epithelium of the retina (red arrow). Panel (L) is a higher magnification view of panel (K). CXCL14 expression is restricted to the ventral medial aspect of the otic vesicle (M). (N) At stage 35, CXCL14 expression persists in the same domains and is also detected in the head mesenchyme (yellow arrows). Lateral view, dorsal to top, anterior to right. (O-R) Transverse sections showing the expression of CXCL14 in the head mesenchyme and the cement gland (O), pigmented epithelium of the retina (O-P) and otic vesicle (Q-R). Panel (Q) corresponds to a section posterior to section shown in panel (R). For comparison at this stage Otx2 (bracket) is expressed in the ventral aspect of the otic vesicle (S) a domain that does not overlap with CXCL14 (R). di, diencephalon; hb, hindbrain; le, lens; ov, otic vesicle; re, retina; st,stomodeum. |
