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Fig. 5. Sox10 is sufficient to induce pigment cell lineage as revealed by Trp-2 expression. (A) Developmental expression of Xenopus Trp-2 by Northern hybridization. The stages are according to Nieuwkoop and Farber (1967). (B) By whole-mount in situ hybridization at stage 27/28, Trp-2 is detected in the developing retina and in individual cells scattered at the dorsal midline at a level posterior to the hindbrain. Dorsal view, anterior to the left. (C) An embryo injected at the two-cell stage with 1 ng of Sox10 mRNA and analyzed at stage 30 displays massive ectopic expression of Trp-2 (left panel), while the uninjected side is unperturbed (right panel). Lateral view, anterior is to the right. (D) RT-PCR analysis of animal explants derived from embryos injected at the two-cell stage with 1 ng of Sox9 or Sox10 mRNA. Only Sox10 overexpression induces strong expression of Trp-2 at stage 33. EF1-alpha is used as a loading control. (E) In situ hybridization of explants overexpressing Sox9, Sox10, or Slug. Induction of Trp-2 is exclusively detected in Sox10-injected animal explants at equivalent stage 32. Trp2 expression in an uninjected embryo (control) at stage 32 is shown (left panel). |
