|
Gene |
Clone |
Species |
Stages |
Anatomy |
dct.L
|
|
laevis
|
NF stage 25
to
NF stage 28
|
neural crest
,
trunk region
,
melanoblast
|
dct.L
|
|
laevis
|
NF stage 32
to
NF stage 35 and 36
|
retina
,
pineal gland
,
neural tube
,
retinal pigmented epithelium
,
melanoblast
|
dct.L
|
|
laevis
|
NF stage 39
|
retina
,
hindbrain
,
head region
,
pineal gland
,
eye
,
[+]
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
mitf
|
|
laevis
|
NF stage 19
|
neural crest
,
trunk neural crest
,
melanoblast
|
ednrb2
|
|
laevis
|
NF stage 21
to
NF stage 25
|
roof plate
,
head region
,
neural crest
,
trunk region
,
neural tube
,
[+]
|
ednrb2
|
|
laevis
|
NF stage 28
|
head region
,
trunk region
,
trunk neural crest
,
neural tube
,
migratory neural crest cell
,
[+]
|
ednrb2
|
|
laevis
|
NF stage 32
|
head region
,
trunk region
,
pineal gland
,
anterior neural tube
,
neural tube
,
[+]
|
ednrb2
|
|
laevis
|
NF stage 35 and 36
to
NF stage 39
|
head region
,
trunk region
,
pineal gland
,
melanophore
,
neural tube
|
|
|
Fig. 1. Ednrb2 and melanoblast marker gene expression patterns in Xenopus embryos by WISH. A,B: Mitf expression is detected (A), but there is no Ednrb2 expression in a stage-19 embryo. C: Ednrb2 expression (arrowheads) is first detected in the NT at stage 21. D–F: Weak Dct (D) and strong Ednrb2 (E,F) expression is detected at stage 25. G–I: Dct (G) and Ednrb2 (H,I) expression is detected in the NT and only Ednrb2 is expressed in the head region at stage 28 (red arrowheads). J–R: The expression pattern of Ednrb2 (K,O,R) resembles that of Dct (J,M,P) except in the RPE (Dct only). Blue arrowheads indicate expression in the epiphysis. Note that after stage 35, melanophores are pigmented, but because the embryos are bleached in these embryos, the melanins are not visible. Insets are magnified images in the squares (scale bar ¼ 100 mm) and show dendritic pigmented melanophores expressing both Dct (P) and Ednrb2 (R). F, I, L, O, and R are lateral views of E, H, K, N, and Q, respectively. Heads are on the right side of each picture. Scale bar ¼ 1 mm. |
|
Display additional annotations [+]
|
|
Fig. 4. Expression patterns of ET3. A,C: ET3 is expressed in the rostral trunk region, where melanophores later migrate to the yolk at stages 25 (A) and 31 (B). B,D: ET3 is expressed in thecaudal trunk region where melanophores later localize in the NT. E,F: Dct expression represents
migrating melanoblasts. E and F are serial sections of C and D, respectively. In the rostral region, melanoblasts seek yolk migrating through the ventral pathway (E). In the caudal region, melanoblasts localize in the NT or migrate to the fin (F). Scale bar ¼ 100 mm. |
|
Display additional annotations [+]
|
|
Fig. 2. Expression patterns of Ednrb2 and Dct A–D: Serial sections of an embryo at stage 26.
Dct-expressing cells also express Ednrb2 in the rostral trunk region (A, B) and in the caudal
trunk region (C, D). A melanoblast initiating migration is observed in A, B (arrowheads). E,F:
Both Dct (E) and Ednrb2 (F) are expressed in the same cells in serial sections of the trunk region
at stage 32. G,H: RPE cells express Dct (G) but not Ednrb2 (H) in serial sections of the eye at
stage 32. Note that the brown color of H in the RPE is not the ISH signal but is due to the melanin.
Scale bar ¼ 100 mm. |