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Figure 1. Effects of SoxE and Mitf isoforms on Dct expression in Xenopus embryos and animal caps. (A) Schematic of SoxE and Mitf proteins and SUMOylation mutants. (B) In situ hybridization showing Dct expression in embryos injected with SoxE2KR, Mitf2KR, or both. Both Sox92KR and Sox102KR act synergistically with Mitf2KR to induce premature and ectopic Dct expression in stage 23 and stage 28 embryos. (C) Animal cap assay showing effects of Sox92KR/SUMO on Mitf2KR-mediated induction of Dct at stage 28. Sox92KR enhances, whereas Sox92KR/SUMO blocks, Dct induction. Light red staining represents lineage tracer β-gal. Bars, 200 µm. AD, activation domain; LZ, Leucine zipper. |