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dkk1xenopus   

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Experiment details for dkk1

Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation.

Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation.

Gene Clone Species Stages Anatomy
dkk1.S laevis NF stage 10 dorsal marginal zone
dkk1.S laevis NF stage 10.5 to NF stage 11 involuted dorsal mesoderm
dkk1.L laevis NF stage 12 prechordal plate
dkk1.S laevis NF stage 13 forebrain , anterior neural tube

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  Figure 2. Tiki1 Expression and Regulation in Early Embryos(A) RT-PCR revealed that Tiki1 mRNA is detectable from stages 9 to 30.(B) Tiki1 is expressed dorsally. Embryos were cut into animal, vegetal, dorsal, and ventral parts for RT-PCR. Xnr3: a positive control.(C–L) Whole-mount in situ hybridization of Tiki1 and Dkk1 during gastrulation. Drawing is on right. (C and D) Vegetal view, dorsal on top; (E and F) hemisection, dorsal on right; (G–L) dorsal view, anterior on top.(M) Tiki1 expression depends on maternal Wnt signaling. Con: control, UV: ultraviolet.