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Figure 2. Tiki1 Expression and Regulation in Early Embryos(A) RT-PCR revealed that Tiki1 mRNA is detectable from stages 9 to 30.(B) Tiki1 is expressed dorsally. Embryos were cut into animal, vegetal, dorsal, and ventral parts for RT-PCR. Xnr3: a positive control.(C–L) Whole-mount in situ hybridization of Tiki1 and Dkk1 during gastrulation. Drawing is on right. (C and D) Vegetal view, dorsal on top; (E and F) hemisection, dorsal on right; (G–L) dorsal view, anterior on top.(M) Tiki1 expression depends on maternal Wnt signaling. Con: control, UV: ultraviolet. |