|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
wnt4
|
|
laevis
|
NF stage 28
|
hindbrain
,
midbrain-hindbrain boundary
,
cranial ganglion
,
heart
,
pharyngeal arch
,
[+]
|
jag1
|
|
laevis
|
NF stage 28
|
brain
,
midbrain
,
hindbrain
,
hyoid crest
,
notochord
,
[+]
|
|
|
Fig. 9. rfng promotes expression of wnt4 and components of the Notch pathway. (A-C) X. laevis embryos were injected as previously described and cultured to stage 28, with beta-galactosidase mRNA co-injected to act as a lineage tracer (red staining on injected side). Ectopic wnt4 (A), delta1 (B) and serrate1 (C) expression was detected after rfng mRNA injection. Asterisk denotes injected side. (D) A model for early stage proximal pronephrogenesis regulated by the Notch signalling pathway. We propose a pool of cells undergoing Notch signalling and located on the lateral side of the dorso-anterior pronephros anlagen at tail bud stages of development, mediate medio-lateral patterning, permitting the glomus and tubules to develop independently. Blue arrows indicate proposed genetic hierarchy; red arrows indicate directional secretion of Wnt4. See text for full description. |