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Fig. 3. (A) Wholemount in situ hybridization of XDLL-1 cRNA to stage 18 embryo visualizes expression of this gene in the cement gland (arrow), This is a lateral view of the embryo. (B) Anterior view of the same embryo. (C) Lateral view of stage 24 embryo demonstrates the expression of XDLL-1 in the neural crest derived visceral arches. 1, cement gland; 2, mandibular arch; 3, hyoid arch; 4, anterior branchial arch; 5, posterior branchial arch. Triangle points to the otic vesicle. (D) Anterior view of the same embryo as in (C), In this view the cement gland and the mandibular arch show strong hybridization. Arrow points to the hybridisation signal in the lateral diencephalon. (E) In situ hybridization to stage 30 embryo (lateral view), At this stage in addition to the cement gland and visceral arches, the diencephalon (arrow) shows strong hybridization. (F) A dorsal view of the same embryo as in (E) shows the segment-like hybridization of XDLL-1 to the visceral arches in the head region. The posterior part of the embryo in (E) and (F) has been removed. |
