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Fig. 1. Xrx1 expression in the proliferative region of the anterior neural plate is controlled by Hedgehog and neurogenin signaling. (A,B) Expression of Xrx1 (light blue) in relation to the expression of (A) X-Delta-1 (purple) and (B) X-ngnr-1 (purple) in stage 13 embryos; frontodorsal views. (C) Sagittal section of a stage 13 embryo showing Xrx1 expression in the deep sensorial layer of the neuroectoderm. (D,E) Stage 14 embryos injected with X-chh (D) and X-shh (E) showing ectopic expression of Xrx1 (blue); frontal views, dorsal towards the top. (F,G) Embryos injected with X-ngnr-1 displaying repression of Xrx1 (blue, F, stage 13) and ectopic expression of N-tubulin (blue, G, stage 16); frontodorsal views. The injected side of the embryos (to the right of vertical bars representing the midline) is indicated (inj). Red staining represents expression of co-injected lacZ lineage tracer. d, deep neuroectodermal layer; s, superficial neuroectodermal layer. |
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Fig. 2. Xrx1 inhibits neurogenesis. The effects of Xrx1 overexpression on (A) X-ngnr-1 (stage 12), (B,D,E) X-Delta-1 (stage 13), (C) N-tubulin (stage 16), (F) Sox2 (stage 13) and (G) XBF-1 (stage 13) are shown. (D,E) Transverse sections at the level of the anterior (D) and posterior (E) neural plate. The black arrowhead indicates the anterior expression domain of X-Delta-1, while the white arrowhead indicates the repression of the corresponding domain in the injected side. (H) Stage 16 embryo injected with 150 pg of XBF-1 RNA showing suppression of endogenous N-tubulin (white arrowheads) as well as ectopic N-tubulin activation at the border of the injected area (arrow). A-C,F,G,H Frontodorsal views. (I,K) Stage 16 embryos co-injected with either X-ngnr-1 and lacZ (I) or X-ngnr-1, Xrx1 and lacZ (K); lateral views, anterior towards the left. (J,L) Animal caps co-injected with either X-ngnr-1 and lacZ (J) or X-ngnr-1 and Xrx1 (L) analyzed at stage 16. Both in whole embryos and in animal caps Xrx1 inhibits N-tubulin expression induced by X-ngnr-1. Red staining represents expression of co-injected lacZ lineage tracer. |
