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Fig. 5. Xiro3 blocks the differentiation of the primary neurons and expands the expression of XASH3. All embryos are viewed from the dorsal side except in (B) (lateral view). In (A and G), embryos are viewed with the anterior end toward the right. The injected side of the embryos is marked by blue staining for nuclear LacZ expression.
(A) Stage 15 embryo injected with Xiro3 mRNA. Note that the lateral stripe of N-tubulin expression is suppressed on the injected side. (B) Stage 15 embryo injected with Xiro3 mRNA showing disorganized N-tubulin expression at the border of the injected area. (C) Stage 15 embryo injected with Xiro3 mRNA showing that the lateral and intermediate stripes of N-tubulin expression are moved to a more ventral position. (D) Stage 14 embryo injected with Xiro3 mRNA. Expression of X-MyT1 is blocked on the injected side of the embryo. (E) Section through the hindbrain region of a stage 30 embryo labelled for N-tubulin expression. (F) Cross-section through a stage 30 embryo injected with Xiro3 in the lateral ectoderm. Note that Xiro3 induces extra tissue in the lateral epidermis of the embryo that is partially N-tubulin positive.
(G) Stage 14 embryo injected with Xiro3 showing ectopic expression of X-Delta-1 over the injected area. (H) Stage 14 embryos injected with a combination of Xiro3 and a dominant-negative form of X-Delta-1, X-Delta-1stu that block lateral inhibition. No rescue of the blocking of N-tubulin expression is observed. (I) Control embryos injected with X-Delta-1stu alone showing an increase of density of N-tubulin-positive cells within the stripes. (J) Stage 16 embryos injected with Xiro3. The expression of XASH3 is slightly expanded on the injected side. |
