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Fig. 8. Overexpression of Evi1-VP16-hGR disrupts distal and expands early proximal markers. (A, I, O) Lateral views and (G, H, M, N and Q, R) sections through the pronephros of tailbud or early tadpole stage embryos coinjected with Evi1-VP16-hGR mRNA and β-galactosidase mRNA and stained with the indicated markers. Control and injected sides marked with Red-gal are shown. Note the inhibition of the expression of the expression of the XClC-K (A, B) and Gremlin (C, D) distal tubule and duct markers (arrowheads), the expansion of the xWT1 (E) and nephrin (I, J) glomus and X-Delta-1 (K) and EphA4 (O, P) early proximal markers (arrows). (Q) Example of injected embryos with reduced proximal tubules. (R) Example of an embryo with a pronephros containing additional tubule branches and ectopic xPDZK1 staining (arrow). Abbreviations: pn, pronephros; spm, splanchnic layer of the mesoderm; pt, proximal tubules. |