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FIG. 3. Comparison of Xath5 expression pattern with that of neurogenic and achaete-scute-like genes in stage 40 Xenopus retina. (A) Comparison of X-Notch-1 and Xath5 expression patterns. Double in situ hybridizations were performed as in Fig. 1 with Xath5 in deep purple and X-Notch-1 in red. (A) Xath5 expression is detected in the CMZ except in cells in the most peripheral region. Occasional labeled cells were seen in the central retina, being probably late born cells. (B) X-Notch-1 labeling is also detected in the CMZ except in cells in the most peripheral region. Occasional labeled cells are seen in the central retina. C and D show a higher magnification of the CMZ from the sections depicted in A and B, respectively. (E) Double labeling demonstrates that X-Notch-1 and Xath5 expressions do not exactly overlap, since some X-Notch-1 positive cells do not express Xath5 (arrow). Scale bar in A and B, 100 um; scale bar in C, D, and E, 30um. (F) Comparison of X-Delta-1 and Xath5 expression patterns. Double in situ hybridizations were performed as in Fig. 1 with Xath5 in deep purple and X-Delta-1 in red. (F) Xath5 expression is detected in the CMZ except in cells in the most peripheral region. (G) X-Delta-1 labeling is also detected in the CMZ except in cells in the most peripheral region. H and I show a higher magnification of the CMZ from the sections depicted in F and G, respectively. (J) Double labeling demonstrates that X-Delta-1 and Xath5 expressions do not exactly overlap, since some X-Delta-1 positive cells do not express Xath5 (arrow). Scale bar in F and G, 100 um; scale bar in H, I, and J, 30 um. (K) Comparison of Xath5 and Xash3 expression patterns. Double in situ hybridizations were performed as in Fig. 1 with Xath5 in deep purple and Xash3 in red. (K) Xath5 expression was restricted to the more central part of the CMZ. (L) Xash3 labeling was also restricted to the more central part of the CMZ. M and N show a higher magnification of the CMZ from the sections depicted in K and L, respectively. (O) Double labeling demonstrates that Xash3 expression starts more in the periphery of the CMZ than Xath5 expression and that there is a small area in the CMZ where both expressions overlap (arrow). Scale bar in K and L, 100 um; scale bar in M, N, and O, 30 um. |
