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dll1xenopus   

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Experiment details for dll1

Beck CW and Slack JM (1998) Assay

Analysis of the developing Xenopus tail bud reveals separate phases of gene expression during determination and outgrowth.

Gene Clone Species Stages Anatomy
dll1.L laevis NF stage 14 neuroectoderm , pre-chordal neural plate , mesoderm , blastopore lip , tail bud , [+]

  dll1 (delta-like 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 14, dorsal view, anterior left.

Gene Clone Species Stages Anatomy
dll1.L laevis NF stage 29 and 30 posterior wall of neurenteric canal , distal , neural tube , tail tip

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  Fig. 4. Early genes expressed in the posterior wall of the tail bud. (A,C) Sagittal sections of stage 30 whole mount in situ hybridisations. Scale bar, 250 mm. (A) Expression of Xbra (dark staining) in the posterior wall and chordoneural hinge, with highest levels distally. (C) Expression of X-delta- 1 in the posterior wall, with highest levels distally. X-delta-1 transcripts are absent from the dorsal roof but punctate expression is seen more anteriorly in the neural tube. (B,D) Schematic diagrams of expression based on the sections shown in (A,C), respectively. (E,F) Whole mount in situ hybridisations showing the expression of other genes in the Notch/delta pathway in the tail bud. (E) X-delta-2 expression is primarily in the most posterior somite (*) but is also weakly expressed in the posterior wall. As for Xdelta- 1, expression is lacking from the dorsal roof of the tail bud. (F) XNotch- 1 is expressed widely in the tail bud but is excluded from the same region of the dorsal roof as X-delta-1 and X-delta-2. All figures are oriented with posterior to the right and dorsal upwards. Abbreviations: cnh, chordoneural hinge; end, endoderm; nc, notochord; nt, neural tube; pw, posterior wall.

Gene Clone Species Stages Anatomy
dll1.L laevis NF stage 29 and 30 fin , brain , forebrain , midbrain , hindbrain , [+]

  dll1 (delta-like 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 29 & 30, lateral view, anterior left, dorsal up.

Gene Clone Species Stages Anatomy
dll1.L laevis NF stage 29 and 30 mesoderm , posterior wall of neurenteric canal , tail , tail tip

  dll1 (delta-like 1) gene expression in Xenopus laevis embryo, tail region only, assayed via in situ hybridization, NF stage 29 & 30, lateral view, anterior left, dorsal up.