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Fig. 4. (A) Whole-mount in situ hybridization to a stage 32 embryo (anterior view) showing the most intense area of XDLL-1 expression in the forebrain - the lateral diencephalic region (arrow) (B) In situ hybridization of XDLL-1 to a stage 33/34 embryo (anterior view), At this stage the entire ventral forebrain is uniformly labeled. (C) A lateral view of the embryo in (B) showing the boundaries of XDLL-1 hybridization within the brain. Upper arrow (e) points at the epiphysis which delineates the dorsal limit of the telencephalon. The lower arrow (d) points at the dorsal limit of XDLL-1 expression boundary in the telencephalon which is ventral to the epiphysis. O, otic vesicle, upper triangle points to the plane of section in (H), lower triangle points to the plane of section in (G). (D) In situ hybridization of XDLL-1 to stage 42 brain. Lateral view. Forebrain is to the left. t, telencephalon; d, diencephalon; i, infundibulum. Apparent signal in the hindbrain area is due to pigment granules and not to hybridization. (E) Wholemount hybridization to stage 43 brain. Arrows indicate the plane of section in (F). (F) Cross section of the telencephalic region of the brain in (E) Notice the sharp boundaries of labeling. (G) Coronal section of the embryo in (c) at the level of pharyngeal arches (lower triangle in (C)). 1, ectoderm; 2, neural crest; 3, mesoderm; 4, endoderm. (H) Coronal section of the embryo in (C) at the level of the eyes (upper triangle in (C)). d, diencephalon; 1, lens; o, otic vesicle; r, retina; t, telencephalon. Notice the presence of hybridization in the retina and the absence of it in the lens. |
