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Fig. 1. Pattern of NECs, and X-Delta-1
expression, in Xenopus embryos following
ectopic expression of XASH-3, NeuroD,
ICD and X-Delta-1Stu. Each panel shows a
dorsal view of a Xenopus embryo at stage
13 (neural plate stage) after staining for Xgal
(sky blue), which reveals the injected
side, and for N-tubulin RNA (dark purple)
which reveals the pattern of NECs
(A,B,D-I), or for X-Delta-1 (C). Each
embryo is oriented with anterior to the left
and posterior to the right, with the injected
side facing down. (A) Ectopic expression
of XASH-3 RNA (1.0 pg) results in a
suppression of NECs. The normal three
strips of NECs are marked on the upper
side: (m) medial, which give rise to
primary motorneurons; (i) intermediate,
which gives rise to primary interneurons;
and (l) lateral, which gives rise to primary
sensory neurons. (B) Ectopic expression
of XASH-3 (0.25 pg) results in ectopic
NECs in a salt and pepper pattern.
(C) Ectopic expression of XASH-3 (2 pg)
results in activation of X-Delta-1
expression. (D) Ectopic expression of
XASH-3 (1 pg) and X-Delta-1Stu (1 pg)
results in an ectopic and dense pattern of
NECs. (E) Lateral, ectopic expression of X-Delta-1Stu (1 pg) results in more NECs in the lateral stripe, but the size of the stripe is largely
unchanged. (F) Ectopic expression of NeuroD (0.5 pg) promotes ubiquitous and dense pattern of NECs. (G) Ectopic expression of NeuroD (0.5
pg) and an activated from of Notch ICD (0.5 pg) gives a similar pattern of NECs as with NeuroD alone. (H) Ectopic expression of ICD (0.5 pg)
blocks the formation of NECs. (I) Ectopic expression of ICD/XASH-3/X-Delta-1Stu (0.5/1.0/1.0 pg) RNA results in a suppression of NECs. |
