| |
Fig. 4 (Left). Spatial expression of arid5b during Xenopus development. In situ hybridization
of whole (A-D, G-L) or transverse fractured embryos (E,F) at the indicated stages of development
with antisense probe for arid5b (nucleotide 775 to 1612 of isoform1) (A,B,D,E,H,J,L,I),
arid5b
arid5b
arid5b sens arid5b-slc12a1
arid5b slc12a1 arid5b-slc12a1
arid5b
arid5b
delta1
delta1
arid5b-slc12a1
st 22 st 25 st 25
st 31 st 25 st 25
st 35/36 st 35/36
st 35/36
st 35/36 st 35/36 st 35/36
G
B C
D E F
H
I
J K L
A
delta1 (C,F), slc12a1 (I,K,L) and with control sense probe for arid5b (G). Lateral views with anterior to the right (A-D,G,H,J-L). Transverse section at
the level of the proximal pronephric tubule. In I and L, arid5b is revealed in light blue and slc12a1 in purple. Arid5b mRNAs are detected in the anterior
pronephric anlage during tailbud stages in a broader domain than delta1. At tadpole stage 35/36, arid5b expression is restricted to the proximal part
of the tubule and does not overlap with slc12a1 expression which is specific for the intermediate and the first segment of the distal tubule. Scale bars
are 0.3 mm. |
