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dll1xenopus   

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Experiment details for dll1

Identification of target genes for the Xenopus Hes-related protein XHR1, a prepattern factor specifying the midbrain-hindbra...

Identification of target genes for the Xenopus Hes-related protein XHR1, a prepattern factor specifying the midbrain-hindbrain boundary.

Gene Clone Species Stages Anatomy
dll1.L laevis NF stage 13 midbrain , hindbrain , neuroectoderm , neural plate , neural plate border , [+]

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  Fig. 4. The effects of XHR1-VP16-GR on the expression of possible target genes and neuronal markers. (A) ESR1, ESR9, XLCL2, Xdelta1, Xngnr1, and XHR1 are activated by XHR1-VP16-GR in the presence of CHX. Embryos were injected into the dorsal left blastomere at the four-cell stage with XHR1-VP16-GR mRNA (100 pg), and treated with or without CHX for 30 min before the addition (or not) of DEX at the gastrula stage (stage 11), as indicated. The expression of ESR1 (a), ESR9 (b), XLCL2 (c), Xdelta1 (d), Xngnr1 (e), and XHR1 (f) were analyzed at stage13 by WISH. (B) The effects of XHR1-VP16-GR on the expression of neuronal markers. The expression of Xlim3 (a), Xash3 (b), and β2-tubulin (c) was analyzed at stage 13 by WISH. Fractions indicate the proportion of the presented phenotype per total number: numbers in black, normal expression; numbers in red, upregulation; numbers in green, disrupted expression; numbers in blue, downregulation. Black arrows in Ad,e, pre-MHB region; red arrow in Ba, ectopic expression; black arrow in Bb, reduced expression.