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dll1xenopus   

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Experiment details for dll1

Olguín P et al. (2006) Assay

RE-1 silencer of transcription/neural restrictive silencer factor modulates ectodermal patterning during Xenopus development.

Gene Clone Species Stages Anatomy
dll1.L laevis NF stage 14 neural plate , neural tube , neural plate border , anterior placodal area , anterior neural ridge , [+]

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  Figure 2. Loss of XREST/NRSF function disturbs neural patterning. Dorsal views of stage 15 embryos injected unilaterally with 500 pg of dnXREST. Anterior is down, and the injected side is to the left. The stage of dexamethasone addition is indicated on top, and probes used for ISH are indicated to the left. Embryos induced at stage 9 [Dex(+) st. 9] displays inhibition of N-tubulin, X-ngnr-1, and X-Delta-1 (arrowheads) and ventral displacement of neurogenic domains (brackets). Induction at stage 12 [Dex(+) st. 12] results in subtler effects. In contrast, embryos induced at both stages exhibit a precocious and ectopic expression of NaV1.2 at stage 14 (arrowheads). Induction of dnXREST at stage 9 associates the expansion of non-neurogenic domain (brackets) marked by Zic2 and surrounded by X-Delta-1 (arrowheads). Activation of dnXREST at stage 12 did not result in the expansion of Zic2.