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Fig. 2. Ectopic expression of the HoxD1 antimorph protein knockdown inhibits primary neuron and neural crest markers in addition to hindbrain markers. (A) Two-cell stage albino embryos were injected unilaterally into the animal hemisphere of one blastomere with 100 pg of RNA encoding the HoxD1 antimorph protein. All embryos are injected on the right side, viewed dorsally and are oriented anterior (top), posterior (bottom). (a) Krox20 expression is inhibited in 87% of the embryos (n = 83/95). (b) N-tubulin expression is inhibited in 97% of the embryos (n = 57/59). (c) Slug expression is inhibited in 85% of the embryos (n = 44/52). (d) FoxD3 expression is inhibited in 87% of the embryos (n = 52/60). (e) Pax3 expression is normal in 100% of the embryos (n = 16/16). (f) Zic1 expression is normal in 93% of the embryos (n = 13/14). XMeis3 expression (not shown) is normal in 100% of the embryos (n = 16/16). (B) Ectopic HoxD1 expression rescues n-tubulin expression in XMeis3 morphant embryos. The dashed red lines mark the dorsal midline. (a) Uninjected control embryo. (b) 18 ng XMeis3 MO, n-tubulin expression (right side) was inhibited in 68% (n = 22) of the embryos. (c) 18 ng XMeis3 MO, 0.8 ng HoxD1 RNA, n-tubulin expression was highly rescued (left side) in 75% (n = 12) of the embryos. |
