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Fig. 6. Pax3 knockdown inhibits primary neuron and hindbrain markers in addition to neural crest marker expression. Two-cell stage albino embryos were injected unilaterally into the animal hemisphere of one blastomere with 20 ng of Pax3 MO. All embryos are viewed dorsally and are oriented anterior (top), posterior (bottom). In each embryo trio panel, the control embryo is on the left, Pax3 mis-match MO injected (mm) center, and the Pax3 MO (MO) on the right. The red arrows mark the dorsal midline. (A) In the shown experiment, XMeis3 expression was inhibited in 76% of the Pax3 morphant embryos (n = 21). Expression was normal in uninjected controls (91%, n = 23) and embryos injected with a 5 bp mm Pax3 MO (90%, n = 22). In all experiments, XMeis3 expression is inhibited in 83% of the morphant embryos (n = 38). (B) In the shown experiment, Slug expression is inhibited in 95% of the morphant embryos (n = 21). Slug expression was normal in uninjected controls (85%, n = 20) and embryos injected with a 5-bp mm Pax3 MO (76%, n = 17). In all experiments, Slug expression is inhibited in 93% of the morphant embryos (n = 55). FoxD3 expression is inhibited in 79% of the morphant embryos (n = 33, not shown). (C) In the shown experiment, n-tubulin expression was inhibited in 78% of the embryos (n = 18) Expression was normal in uninjected controls (81%, n = 21) and embryos injected with a 5-bp mm Pax3 MO (75%, n = 24). In all experiments, n-tubulin expression is inhibited in 90% of the morphant embryos (n = 58). (D) In the shown experiment, Krox20 expression is inhibited in 95% of the morphant embryos (n = 21). Expression was normal in uninjected controls (76%, n = 21) and embryos injected with a 5-bp mm Pax3 MO (74%, n = 19). In all experiments, Krox20 expression is inhibited in 93% of the morphant embryos (n = 133). (E) In the shown experiment, c-myc expression was normal in morphant embryos (86%, n = 22). Expression was normal in uninjected controls (88%, n = 24) and embryos injected with a 5 bp mm Pax3 MO (77%, n = 22). In all experiments, c-Myc expression is normal in 92% of the Pax3 morphant embryos (n = 38). (F) Sox2 expression was normal in morphant embryos (76%, n = 23). Expression was normal in uninjected controls (91%, n = 23) and embryos injected with a 5-bp mm Pax3 MO (82%, n = 22). |
