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Fig. 4. Spatial expression pattern of XrhoB during early neurula stages. (A–B) Stage 14. (A) Dorsal view. (B) Anterior view. (C–D) Transverse paraffin sections across the C and D lines shown in (A) and (B). In (C), the black arrowhead shows RhoB staining in posterior paraxial mesoderm and the white, in axial tissue. (E) XrhoB expression pattern at stage 16. Dorsal view. XrhoB is expressed in the developing central nervous system, both in the forebrain and the hindbrain and accumulates in specific rhombomeres. (F) Stage 16 embryos hybridized with XrhoB (purple) and Xkrox20 (red) antisense probes. Dorsal view. Krox20 is expressed in rhombomeres 3 and 5 (R3 and R5) and in the neural crest cells (nc) adjacent to R5. In the hindbrain, expression of XrhoB accumulates in R4, based on the expression domains of krox20, and in a rhombomere above R3 (white arrowhead) that may correspond to R1 or R2 based on distances between streaks. (G) Xtwist staining at stage 16. Dorsal view. Xtwist is expressed in neural crest cells at the lateral edges of the neural plate. (H) XrhoB expression at stage 16. Dorsal view. In the trunk region, XrhoB is expressed in the neural tube and the developing somites. (I) XrhoB staining at stage 22. Dorsal view. (J) Transverse paraffin section across the J line of the embryo shown in (I). XrhoB is expressed in the dorsal part of the neural tube and in cranial migrating neural crest cells. (K) Xtwist staining at stage 22. Dorsal view. (L) Transverse paraffin section across the L line of the embryo shown in (K). Abbreviations: anr, anterior neural ridge; for, forebrain; hb, hindbrain; mnc, migrating neural crest cells; nc, neural crest cells; no, notochord; nt, neural tube; R3, rhombomere 3; R4, rhombomere 4; R5, rhombomere 5; so, somites. |
