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Fig. 6. Depletion of XTcf-1 (Tcf1Mo), XTcf-3 (Tcf3Mo) and XLef-1 (Lef1Mo) have different effects on XTcf-4 expression (A) and en2 expression (C). 4 pmol of the indicated morpholino antisense oligonucleotides was injected into one blastomere of two-cell stage embryos and stained for the expression of XTcf-4 (A) and en2 (C). The asterisk marks the injected site. B and D show the quantification of reduced marker gene expression for different amounts of XTcf-1 morpholino injections. n: number of analyzed embryos. |