|
|
|
Fig. 3. Wholemount in situ hybridisation of XEZ. Wholemount in situ hybridisation of XEZ DIG labelled antisense RNA probe shows that expression of XEZ is restricted to the anterior nervous system. (A) Stage 14 embryo expression is restricted to the anterior nervous system (indicated by arrow) (B) Stage 20 embryo and (C) Stage 27 embryo, the XEZ transcripts are detected in the anterior nervous system. In (B) and (C) the expression pattern appears to be graded with the strongest expression in the most anterior regions. (D) Stage 35 embryo shows that expression of XEZ later becomes restricted to the forebrain, eye and branchial arches. The XEZ expression is highest in the first branchial arch, less in the second and is greatly reduced in the third and fourth arches. The sense probe control showed no staining pattern (data not shown). |