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fgf8xenopus   

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Experiment details for fgf8

Lupo G et al. (2005) Assay

Dorsoventral patterning of the Xenopus eye: a collaboration of Retinoid, Hedgehog and FGF receptor signaling.

Gene Clone Species Stages Anatomy
fgf8 xenopus NF stage 17 anterior neural fold
fgf8 xenopus NF stage 17 to NF stage 18 midbrain-hindbrain boundary , neuroectoderm , anterior neural fold , neural tube

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  Fig. 4. Expression pattern of Raldh3 during Xenopus development. (A) Raldh3 expression as detected by whole-mount in situ hybridization of whole embryos (stage 10.25-31) or dissected neural retinas, without lens and pigmented epithelium (stage 59). cf, choroid fissure. Scale bar: 350 μm. (B) Raldh3 expression in transverse sections of stage 33 embryos after whole-mount in situ hybridization. From left to right, sections show expression at progressively posterior levels. op, olfactory placode; os, optic stalk. (C) Comparison of Pax2, Raldh3 and Vax2 expression in the optic cup in transversal sections of stage 33 embryos after whole-mount in situ hybridization. (D) Double in situ hybridizations of Rx1 with Raldh2, Shh or FGF8 on mid- to late neurula embryos shown from anterior view. The inset shows a double in situ hybridization of Rx1 with FGF8 on an early neurula embryo.

Gene Clone Species Stages Anatomy
fgf8 xenopus NF stage 18 ectoderm , midbrain-hindbrain boundary , neuroectoderm , anterior neural fold , lateral plate mesoderm
fgf8 xenopus NF stage 33 and 34 olfactory placode , otic vesicle , retina , midbrain-hindbrain boundary , pharyngeal arch , [+]

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  Fig. 9. RA, Hh and FGFR signaling pathways can crossregulate each other at the transcriptional level. (A) High dose RA treatments (10 μM) strongly repress Shh expression in the anterior midline (indicated by the yellow bracket) both at neurula and stage 33. The same RA doses cause fusion of the two stripes of FGF8 expression in the ANR and the anteroventral ectoderm in neurula stage embryos, while at stage 33 FGF8 expression is strongly downregulated in the whole head region. Low dose RA treatments (0.1 μM) have weaker effects on both Shh and FGF8. (B) Unilateral injection of 500 pg bhh mRNA laterally expands FGF8 expression in the anterior neural ridge but does not affect Raldh2 expression at neurula stages. The inset shows FGF8 expression in the ANR of an uninjected embryo at the same stage. The same dose of bhh upregulates both FGF8 and Raldh2 in the eye region at stage 33. (C) Unilateral injection of 4 pg iFGFR1 mRNA, followed by induction with AP20187 at stage 12.5/13, expands Shh expression in the prospective hypothalamic region at neurula, and activates Shh in the eye regions at stage 33. At doses of 4 and 2 pg, iFGFR-1 mRNA has no significant effect on Raldh2 expression at neurula stages and stage 33, respectively.