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Fig. 5. XTcf-4 regulates the expression of midbrain and isthmus marker genes. Ten picomoles of XTcf-4 morpholino were coinjected with 100 pg EGFP-RNA. At late neurula stages, embryos were sorted by the fluorescence signal of the coinjected EGFP. At stage 30, embryos were fixed and stained by in situ hybridization for the expression of the indicated genes. The midbrain marker genes wnt-2b, otx-2, and pax-6 and the isthmus marker genes en-2 and fgf-8were markedly reduced at the XTcf-4 morpholino (Tmo)-injected side (marked with an asterisk), while the forebrain marker bf-1 and the rhombomere 3 and 5 marker krox-20 were not reduced. The control morpholino (Como) did not reduce the expression of any of the marker genes analyzed. In early neurula (stage 13), the XTcf-4 morpholino did not alter the expression of pax-6. f: forebrain, m: midbrain, h: hindbrain. |
