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Fig. 4. Xhex MO knockdown reduces anterior development similar to removing the SBE. Embryos are injected with the Xhex MO and DMZs are isolated and
cultured to tadpole stages (A). Uninjected siblings (B) and control MO injected embryos (C) show a wild-type XBF1 expression pattern, while Xhex MOinjected
embryos (D) exhibit an abnormal XBF1 expression pattern. Wild-type (E) and control MO-injected (F) DMZs develop normal anterior character with
wild-type XBF1 expression (96% and 100%, respectively; 72 DMZs counted from three experiments). Xhex MO-injected DMZs do not develop recognizable
anteroposterior axes and exhibit altered XBF1 expression (G and H, 55%; 72 DMZs counted from three experiments). When recognizable, anterior is to left in
all panels. |
