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Fig. 5. Rescue of Xhex MO knockdown by mouse Hex RNA injection. Wild-type XBF1 expression is identical in stage 24 wild-type whole embryos (A) and
DMZ explants (B; 92% of DMZs, n 37). The inset in A shows a higher magnification view of the expression pattern. Xhex MO-injected DMZs (C) show
reduced or no XBF1 expression (results from one experiment shown; on average, 67% have no XBF1 expression, n 33) and rarely develop a wild-type
morphology. DMZs coinjected with the Xhex MO (40 ng) and mouse Hex RNA (D, 100 pg) appear more wild-type in morphology and a normal XBF1
expression pattern can be rescued (41% express XBF1, 59% do not, n 39). |
