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Fig. 6. Knockdown of Xhex function in the animal hemisphere increases the frequency of severe anterior truncations and compromises the anterior-inducing
property of the SBE. Embryos injected animally with the XHexMO (B) are routinely truncated at midbrain or hindbrain levels compared to controls (A). XBF1,
En2 and Krox20 expression is greatly reduced and often absent (43%; n 28). SBEs from these injected embryos lose the ability to induce anterior character
when combined with gastrula stage animal caps as evidenced by the lack of cement gland formation (D). Control MO-injected SBEs retain anterior inducing
abilities (C). |
