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Fig 8. Dsel is expressed in the brainstem in tadpole embryos.
Specimen after whole-mount in situ hybridization are shown in dorsal view (A,A’), lateral view (B,D) and ventral view (C,E). (A,A’) Embryo at stage 36. Arrows label distinct Dsel expression domains in the midbrain and hindbrain. The arrowhead labels the position of the midbrain-hindbrain boundary. (B-E) Isolated central nervous system from stage 43 embryos after removing of surrounding tissues. Dsel mRNA accumulates in bilateral domains in the brainstem (arrows). Expression of Foxg1 demarcates the forebrain and En2 the midbrain-hindbrain isthmus (arrowhead). hb, hindbrain; mb, midbrain; sp, spinal cord. Scale bars are 1 mm (A-E) and 100 μm (A’). |
