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Fig. 2. Identification of Hnf1β and Fzd4 as direct endodermal RA-target genes
(A) Experimental procedure for the identification of early direct RA-target genes in the
context of pancreas development. (B) Venn diagram comparing genes differentially
expressed within two hours after RA-addition in the absence or presence of CHX. 46 putative
direct RA-target genes were induced under both conditions. (C) RNA-sequencing results for
RA-mediated Hnf1β and Fzd4 induction. The number of mapped reads two hours after the
addition of RA in the presence or absence of CHX is indicated. The data result from two
biological replicates with approximately 50 explants per condition. Average values are given
as mean and error bars as s.e.m.. (D) WMISH for Hnf1β and Fzd4 in gastrula stage
embryos. Whole embryos are depicted on the left-hand side and bisected embryos on the
right-hand side. dbl, dorsal blastopore lip; e, endoderm; m, mesoderm; ne, neuro-ectoderm |
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Fig. S5. Embryonic expression of Hnf1β and Fzd4 is RA-dependent
(A) WMISH for Hnf1β and Fzd4 at gastrula stage in untreated, RA-treated and cyp26a1-
injected embryos. Images on the left display whole embryos (dorsal side up) and images on
the right show bisected embryos (dorsal side on the right). dbl, dorsal blastopore lip; e,
endoderm; m, mesoderm; ne, neuro-ectoderm. (B) Nanostring analysis of untreated, RAand
BMS-treated embryos collected at indicates stages. |
