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Fig. 3. Whole-mount in situ hybridization analysis of Xfz4 mRNA expression. (A–C) Dorso-vegetal view. (A) Stage 10.5, Xfz4 mRNA is enriched in the dorsal ectoderm. (B) Stage 11, Expression of Xfz4 mRNA is further restricted to the neuroectoderm. Bl, dorsal blastopore. (C) Stage 12, Xfz4 mRNA is detected in the anterior neuroectoderm. yp, yolk plug. (D,E) Sagittal sections made from (B,C), respectively, showing expression of Xfz4 mRNA in the deep layer of neuroectoderm (arrows). (F) Stage 18. Anterior view shows the expression of Xfz4 mRNA as a band on both side of the forebrain. (G) Stage 18. Anterior-ventral view shows expression of Xfz4 mRNA in the flank region. Notice that the ventral region expresses low level of Xfz4 mRNA. (H) Stage 18. Dorsal view. Expression of Xfz4 mRNA in the flank narrows dorsally. (I) Stage 14 parasagittal section showing expression of Xfz4 mRNA in the thickened anterior neural plate (Arrows). (J) Stage 18 transverse section showing expression of Xfz4 mRNA in the lateral plate mesoderm (arrows). Notice that somites (S) and notochord do not express Xfz4 mRNA. (K) Stage 25. Ventral view. Strong expression of Xfz4 mRNA in the lateral plate mesoderm flanking the heart primordium. (L) Stage 28. Lateral view. Xfz4 mRNA is detected in the forebrain and posterior unsegmented somitic mesoderm. |
