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gadd45axenopus   

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Experiment details for gadd45a

Gadd45a and Gadd45g regulate neural development and exit from pluripotency in Xenopus.

Gadd45a and Gadd45g regulate neural development and exit from pluripotency in Xenopus.

Gene Clone Species Stages Anatomy
gadd45a.S laevis NF stage 10.5 ectoderm , mesoderm
gadd45a.S laevis NF stage 18 ectoderm , neuroectoderm , cement gland primordium , paraxial mesoderm
gadd45a.S laevis NF stage 32 head region , pronephric duct , endoderm , proctodeum , pronephric kidney

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  Fig. 2. Differential expression of Gadd45a, Gadd45b and Gadd45g during Xenopus embryogenesis. (A–C) qPCR analyses showing the mean concentration normalized to GAPDH of Xenopus Gadd45a (A), Gadd45b (B) and Gadd45g (C). (D) Quantification of relative transcript levels of Xenopus Gadd45a, Gadd45b and Gadd45g in tadpole stage (st. 32) embryos. (E–S) Whole mount in situ hybridization expression analyses. (E–H) Gadd45a expression in lateral view of whole (E) and sagitally halved (F) gastrula stage (st. 10.5) embryos, dorsal is right. (G) Anterior and (H) dorsal view of late neurula stage (st. 18) embryo. (I and J) Gadd45b expression in anterior view of stage 18 embryo using antisense (I) and sense control probe (J). (K and L) Gadd45g expression in anterior view (K) and dorsal view (L) of stage 18 embryo. (M–P) Expression analysis of stage 18 embryos cut transversally with the probes indicated. (Q–S) Lateral view of tadpole stage (st. 32) embryos hybridized with probes for Gadd45a (Q), Gadd45b (R) and Gadd45g (S). The cement glands are outlined by dashed lines in (I–K). Key: (ba) branchial arches, (bp) blastopore, (cg) cement gland, (ey) eye, (fb) forebrain, (hb) hindbrain, (ht) heart field, (mes) mesoderm, (mb) midbrain, (nf) neural fold, (np) primary neuron precursors, (nt) neural tube, (pd) proctodeum, (pn) pronephros, (so) somites, (tg) trigeminal placode.